U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX5550863: Whole genome sequencing of Oryza granulata
1 ILLUMINA (Illumina HiSeq 2000) run: 92.5M spots, 18.5G bases, 12Gb downloads

Design: Fresh and healthy leaves were harvested and immediately frozen in liquid nitrogen, followed by the preservation at -80C in the laboratory prior to DNA extraction. High-quality genomic DNA was extracted from leaves using a modified CTAB method. RNase A was used to remove RNA contamination. The quality and quantity of the extracted DNA were examined using a NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and electrophoresis on a 0.8% agarose gel, respectively.
Submitted by: South China Agricultural University
Study: Oryza meyeriana var. granulata Genome sequencing and assembly
show Abstracthide Abstract
Oryza granulata as an upland wild rice species possesses many different traits from other Oryza species, such as tolerance to shade and drought, immune to bacterial blight and resistance to brown planthopper. Within the diploid Oryza species, O. granulata has the second largest genome size (882 Mbp) with a GG genome, only less than O. australiensis (965 Mbp, genome EE), which is about two times the size of the rice genome (420 Mbp, genome AA). This twofold increasing of its size is mainly due to the accumulation of TE, according to the existing research results. Here, we report a de novo assembly and annotation of O. granulate genome using Illumina sequencing and Hi-C data. These data will be expected to provide insights into the dynamics of Oryza genome evolution and divergence, and serve as important genetic resources for rice breeding.
Sample: Plant sample from Oryza granulata
SAMN11160423 • SRS4516407 • All experiments • All runs
Library:
Name: MP01
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 92.5M spots, 18.5G bases, 12Gb
Run# of Spots# of BasesSizePublished
SRR876030192,496,81318.5G12Gb2019-05-01

ID:
7494891

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...